Scientists Insert Metabolic Pathway for Carbon Fixation & Sugar Production Into E. coli Bacterium

“The Institute scientists rose to this challenge by inserting the metabolic pathway for carbon fixation and sugar production (the so called Calvin cycle) into the bacterium E. coli, a known “consumer” organism that eats sugar and releases carbon dioxide. The metabolic pathway for carbon fixation is well known, and Milo and his group reckoned that, with proper planning, they would be able to attach the genes containing the information for building it into the bacterium’s genome. Yet the main enzyme used in plants to fix carbon, RuBisCO, utilizes as a substrate for the CO2 fixation reaction a metabolite which is toxic for the bacterial cells. Thus the design had to include precisely regulating the expression levels of the various genes across this multistep pathway.”

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